Journal: Drug Metabolism and Disposition

Article Title: Wuzhi Tablet ( Schisandra Sphenanthera Extract) Protects against Acetaminophen-Induced Hepatotoxicity by Inhibition of CYP-Mediated Bioactivation and Regulation of NRF2-ARE and p53/p21 Pathways

doi: 10.1124/dmd.114.059535

Figure Lengend Snippet: WZ possesses marked inhibitory effects on the activities of P450 enzymes and NAPQI-GSH formation. (A) Western blot analysis of CYP2E1, CYP1A2, CYP3A11, and glyceraldehyde-3-phosphate dehydrogenase levels in livers from control, WZ (700 mg/kg)-treated, APAP-treated, WZ (700 mg/kg)/APAP-treated mice. (B) Densitometric analysis of Western blots (n = 3). (C) Effect of WZ on the activities of CYP2E1, CYP1A2, CYP3A11 enzymes in mouse liver microsomes (n = 5). (D) Effect of WZ on NAPQI-GSH formation in mouse liver microsomes (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 versus control mice; #P < 0.05, ##P < 0.01 versus APAP-treated mice.

Article Snippet: CYP1A2 and CYP2E1 antibodies were purchased from Boster Biotechnology Co., Ltd. (Wuhang, China).

Techniques: Western Blot, Control

Journal: bioRxiv

Article Title: R-Spondin Mimetic, SZN-043, Induced Proliferation and Wnt Activity, Two Features Deficient in Human Alcohol-Associated Liver Disease

doi: 10.1101/2024.11.19.624277

Figure Lengend Snippet: (A) QPCR expression analysis of CYP1A2 . (B) PSR histological staining and CYP1A2 immunostaining of control and alcohol-associated cirrhosis liver sections.

Article Snippet: A rabbit anti-CYP1A2 antibody from BosterBio (Catalog # PB9545) and a rabbit anti-ASGR1 antibody from ThermoFisher Scientific (Catalog # PA5-80356) were employed.

Techniques: Expressing, Staining, Immunostaining, Control

Journal: bioRxiv

Article Title: R-Spondin Mimetic, SZN-043, Induced Proliferation and Wnt Activity, Two Features Deficient in Human Alcohol-Associated Liver Disease

doi: 10.1101/2024.11.19.624277

Figure Lengend Snippet: (A) Serum exposure of SZN-043 over time after a single intravenous administration of various SZN-043 doses ranging from 0.3 to 200 mg/kg. (B-G) Time course qPCR expression analysis of (B) Cyp1a2 , (C) Axin2 , (D) Notum , (E) Ccnd1 , (F) Mki67 and (G) Alpl and (H) serum ALP level.

Article Snippet: A rabbit anti-CYP1A2 antibody from BosterBio (Catalog # PB9545) and a rabbit anti-ASGR1 antibody from ThermoFisher Scientific (Catalog # PA5-80356) were employed.

Techniques: Expressing

Journal: bioRxiv

Article Title: R-Spondin Mimetic, SZN-043, Induced Proliferation and Wnt Activity, Two Features Deficient in Human Alcohol-Associated Liver Disease

doi: 10.1101/2024.11.19.624277

Figure Lengend Snippet: (A) Study Design. Aging C57BL/6J female mice were acclimated to the Lieber-DeCarli diet for 5 days, followed by an ethanol-supplemented liquid diet for 8 weeks. A control group was pair-fed with an equicaloric diet during this 8-week period. Starting on week 2 of the ethanol-supplemented diet, mice received 20% ethanol by oral gavage twice weekly for the remaining 7 weeks. The pair fed control group received an equivalent volume of ethanol-free equicaloric dose by oral gavage twice weekly also. Ethanol administration was then discontinued, and mice were returned to an ethanol-free liquid diet. The pair-fed control group and an ethanol-fed were euthanized upon ethanol discontinuation, on Day 0, and liver tissue was collected for analysis. Two hours later and daily thereafter until Day 6, mice received either SZN-043 (30 mg/kg), a control antibody anti-GFP (30 mg/kg) or a vehicle control. On Day3 and on Day 7, upon termination, liver tissue was collected for analysis. (B) QPCR expression analysis of Axin2 , Mki67 , Cyp1a2 , Asgr1 and Asgr2 genes. (C) Immunofluorescence staining with the proliferation marker, anti-Ki67, and the hepatocyte-specific marker, anti-HNF4A antibodies. (D) Percentage of double positive HNF4A and Ki67 cells as determined by quantitative image analysis.

Article Snippet: A rabbit anti-CYP1A2 antibody from BosterBio (Catalog # PB9545) and a rabbit anti-ASGR1 antibody from ThermoFisher Scientific (Catalog # PA5-80356) were employed.

Techniques: Control, Expressing, Immunofluorescence, Staining, Marker

Journal: Toxins

Article Title: The Reduction of the Combined Effects of Aflatoxin and Ochratoxin A in Piglet Livers and Kidneys by Dietary Antioxidants

doi: 10.3390/toxins13090648

Figure Lengend Snippet: Relative protein expression and the corresponding quantification of Western blot images for CYP1A2, CYP2E1, CYP3A4 and GSTA1 in the hepatic and renal microsomal fractions of weaned piglets subjected to experimental diets. Calnexin band (70 kDa) was used as reference protein. The control group (C) were fed a basal diet. The experimental groups were fed as follows: the basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal) (E1 group), the basal diet artificially contaminated with AFB1 and OTA (E2 group), and the basal diet containing the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of AFB1 and OTA (E3 group). The data are illustrated as average values of the groups ( n = 4) ± standard deviation of the mean (SE) and statistical significance related to the control group level. * E1/E2/E3 vs. C; # E2/E3 vs. E1; *, # p < 0.05; **, ## p < 0.01; *** p < 0.001.

Article Snippet: Primary antibodies used were rabbit polyclonal antibodies anti-CYP1A2 (MyBioSource, San Diego, CA, USA, MBS9605022, 1:750), anti-CYP2E1 (MyBioSource, San Diego, CA, USA, MBS9605034, 1:750), anti-Cytochrome P450 Enzyme CYP3A4 (Merck, Temecula, CA, USA, AB1254, 1:1000) and anti-GSTA1 (NovusBiologicals, NBP1-33586, Centennial, CO, USA, 1:1000).

Techniques: Expressing, Western Blot, Control, Standard Deviation

Journal: Toxins

Article Title: The Reduction of the Combined Effects of Aflatoxin and Ochratoxin A in Piglet Livers and Kidneys by Dietary Antioxidants

doi: 10.3390/toxins13090648

Figure Lengend Snippet: Enzymatic specific activity in the hepatic and renal microsomal fractions for CYP1A2, CYP2E1, CYP3A29 and GSTA1 of weaned piglets subjected to experimental diets. The control group (C) were fed a basal diet. The experimental groups were fed as follows: the basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal) (E1 group), the basal diet artificially contaminated with AFB1 and OTA (E2 group) and the basal diet containing the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of AFB1 and OTA (E3 group). The data are illustrated as average values of the groups ( n = 4) ± standard deviation of the mean (SE) and statistical significance related to the control group level. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Primary antibodies used were rabbit polyclonal antibodies anti-CYP1A2 (MyBioSource, San Diego, CA, USA, MBS9605022, 1:750), anti-CYP2E1 (MyBioSource, San Diego, CA, USA, MBS9605034, 1:750), anti-Cytochrome P450 Enzyme CYP3A4 (Merck, Temecula, CA, USA, AB1254, 1:1000) and anti-GSTA1 (NovusBiologicals, NBP1-33586, Centennial, CO, USA, 1:1000).

Techniques: Activity Assay, Control, Standard Deviation